\u0627\u0644\u062f\u0643\u062a\u0648\u0631 \u062e\u0627\u0644\u062f \u0635\u0628\u0627\u0631\u0646\u0629 \u0648\u0627\u0644\u0627\u0633\u062a\u0627\u0630 \u0641\u0631\u0627\u0633 \u0627\u0644\u0641\u062d\u064a\u0644\u064a \u064a\u0646\u0634\u0631\u0627\u0646 \u062f\u0631\u0627\u0633\u0629 \u0628\u0639\u0646\u0648\u0627\u0646 Over-Expression, Purification, and Characterization of Plant<\/p>\n
Title: Over-Expression, Purification, and Characterization of Plant<\/p>\n
LeGST01, the Mammalian GST-Omega Homolog that Interacts
\nwith LeTPX1 from a Tomato cDNA Library.<\/p>\n
Abstract<\/p>\n
The study focuses on an in vivo GST- omega homologue (pCRT7\/TPxII intB4) over-expression, purification and characterization.
\nExperiments purport to characterize the antioxidant activity of the LeTPx1, the interacting glutathione S-transferases
\nBI-GST\/GPx, LeGST-T1, T2, T3, T4, T5 and the mammalian inhibitor of apoptosis Bcl-2. Upon specific
\nexpression, the proteins exerted, differential protective effects in yeast cells treated with lethal doses of the prooxidants hydrogen
\nperoxide, t-butyl hydroperoxide, and cumene hydroperoxide. The antioxidant activity of LeTPx1 was highest against
\nthe cumene hydroperoxide. The overexpressing GST (omega) homologue TPxintB4 (Baier and Dietz, 1999) which share a
\nconsiderable homology of the mammalian GST-omega1. In conclusion, the work shows that yeast parental strains are extremely
\nsensitive to very low concentrations (0.2mM) of Cumenehydroperoxide. However, after applying the different antioxidants;
\nit appears that the smallest concentrations t to be tolerated.<\/p>\n
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